Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

Western

Western blot analysis of Phospho-p53 (Ser46) in MCF lysates untreated or treated with Anisomycin using p53 (Phospho-Ser46) antibody.

Immunofluorescence

Immunofluorescence analysis of Phospho-p53 (Ser46) in Hela using p53 (Phospho-Ser46) antibody.