ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.

WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200  FC: 1/50-1/100  ChIP: 1/20  

Western

Western blot analysis of ER alpha in (1) MCF-7 lysates; (2)T47D lysates using Estrogen Receptor alpha antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human cervix carcinoma using ER alpha antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Estrogen Receptor alpha in MCF-7 using ER alpha antibody.

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